Recombinant CD3e Antibody [clone rC3e/1931] (V8202)

IHC staining of FFPE human spleen with recombinant CD3e antibody (clone rC3e/1931). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant CD3e antibody (clone rC3e/1931). These results demonstrate the foremost specificity of the rC3e/1931 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free recombinant CD3e antibody (clone rC3e/1931) as confirmation of integrity and purity.

IHC staining of FFPE human tonsil with recombinant CD3e antibody (clone rC3e/1931). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding.