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Home >> Antibodies >> Recombinant CD3e Antibody

Recombinant CD3e Antibody [clone C3e/3125R] (V7557)

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  Catalog No Formulation Size Price (USD)  
Image V7557-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7557-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7557SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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Western blot testing of human MOLT-4 cell lysate with recombinant CD3e antibody (clone C3e/3125R). Predicted molecular weight ~23 kDa.
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Western blot testing of human MOLT-4 cell lysate with recombinant CD3e antibody (clone C3e/3125R). Predicted molecular weight ~23 kDa.
IHC staining of FFPE human tonsil with recombinant CD3e antibody (clone C3e/3125R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD3e antibody (clone C3e/3125R) as confirmation of integrity and purity.
IHC staining of FFPE human tonsil with recombinant CD3e antibody (clone C3e/3125R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name C3e/3125R
Purity Protein A affinity chromatography
UniProt P07766
Localization Cell surface, cytoplasmic
Applications ELISA (order BSA-free format for coating) :
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant CD3e antibody is available for research use only.
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Description

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Application Notes

Optimal dilution of the recombinant CD3e antibody should be determined by the researcher.

Immunogen

A recombinant human partial protein (amino acids 23-119) was used as the immunogen for the recombinant CD3e antibody.

Storage

Store the recombinant CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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