• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> Recombinant CD34 Antibody

Recombinant CD34 Antibody [clone HPCA1/2598R] (V7384)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image V7384-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
Image
V7384-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
Image
V7384SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Image
V7384IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
Bulk quote request
IHC testing of FFPE human renal cell carcinoma with recombinant CD34 antibody (clone HPCA1/2598R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
›
IHC testing of FFPE human renal cell carcinoma with recombinant CD34 antibody (clone HPCA1/2598R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human colon carcinoma with recombinant CD34 antibody (clone HPCA1/2598R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human angiosarcoma with recombinant CD34 antibody (clone HPCA1/2598R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human breast carcinoma with recombinant CD34 antibody (clone HPCA1/2598R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human tonsil with recombinant CD34 antibody (clone HPCA1/2598R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant CD34 antibody (clone HPCA1/2598R) as confirmation of integrity and purity.
Flow cytometry testing of human Jurkat cells with CD34 antibody (clone HPCA1/2598R); Red=isotype control, Blue= CD34 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name HPCA1/2598R
Purity Protein A affinity chromatography
UniProt P28906
Localization Cell surface, cytoplasmic
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant CD34 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : FACS, IHC-P
    Reactivity : Human, Rat
    Recrabbitmono
  • Applications : WB, IHC
    Reactivity : Human
    Pred. Reactivity : Mouse, Rat
    Recrabbitmono
  • Applications : ELISA, FACS, IF
    Reactivity : Human, Rat
    Citation  Citations(5)
  • Applications : FACS
    Reactivity : Human and Rat. Other species not known.
    Citation  Citations(5)
  • Applications : FACS, IF, IHC-P
    Reactivity : Human
    Citation  Citations(9)
  • Applications : IHC, WB, ELISA
    Reactivity : Human
  • Applications : IHC-P
    Reactivity : Human
  • Applications : FACS, IF, IHC-P
    Reactivity : Human
  • Applications : FACS, IHC-P
    Reactivity : Human
  • Applications : IHC-P
    Reactivity : Human
  • Applications : FACS, IHC-P
    Reactivity : Human
  • Applications : FACS, IHC-P
    Reactivity : Human
  • Applications : WB, IHC-P, IHC-F, FACS
    Reactivity : Human, Mouse, Rat
  • Applications : WB, IHC-P, IF
    Reactivity : Human
  • Applications : WB, FACS, ELISA
    Reactivity : Human
  • Applications : WB, ELISA
    Reactivity : Human
    Pred. Reactivity : Mouse
  • Applications : FACS, WB, ELISA
    Reactivity : Human

Description

This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels >85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.

Application Notes

Optimal dilution of the recombinant CD34 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant full-length human protein was used as the immunogen for the recombinant CD34 antibody.

Storage

Store the recombinant CD34 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Related Products

   1.   View all CD34 antibodies

   2.   View all recombinant antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.