- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
CD34 Antibody for FACS / Flow Cytometry Immunophenotyping Antibody [clone CDLA34-2R] (V7456)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V7456-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
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V7456-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
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V7456SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 | |
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V7456IHC-7ML | Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* | 7 ml | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | CDLA34-2R |
| Purity | Protein A affinity chromatography |
| UniProt | P28906 |
| Localization | Cell surface, cytoplasmic |
| Applications | Flow Cytometry : 0.5-1ug/10^6 cells Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Prediluted IHC Only Format : incubate for 30 min at RT (1) |
| Limitations | This CD34 Antibody for FACS / Flow Cytometry Immunophenotyping Antibody is available for research use only. |
|
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Cluster of Differentiation 34 (CD34) is a transmembrane glycoprotein encoded by the CD34 gene and expressed on hematopoietic stem and progenitor cells as well as early endothelial lineage populations. It functions in cell adhesion and migration and serves as a key marker of immature hematopoietic cells. CD34 Antibody for FACS is widely used in flow cytometry immunophenotyping, where it enables precise identification and characterization of progenitor cell populations within complex and heterogeneous samples.
CD34 antibody, also referred to as hematopoietic progenitor marker antibody or flow cytometry lineage marker antibody, plays a central role in defining early differentiation states in multiparameter FACS analysis. In flow cytometry, CD34 expression distinguishes undifferentiated progenitor cells from mature hematopoietic populations, allowing researchers to map lineage progression and quantify shifts in progenitor compartments under different biological or experimental conditions. The clear separation of CD34-positive populations supports accurate gating and quantitative analysis across diverse sample types including bone marrow aspirates and peripheral blood mononuclear cells.
This CD34 Antibody for FACS is uniquely suited for immunophenotyping applications, where it functions as a foundational marker within complex antibody panels. CD34 is commonly used in combination with markers such as CD38, CD45, CD90, CD117, and CD133 to define discrete progenitor subsets and hierarchical differentiation pathways. These multiparameter panels enable high-resolution analysis of hematopoietic systems and support detailed characterization of immune development, progenitor expansion, and lineage commitment.
Clone CDLA34-2R is a recombinant rabbit monoclonal antibody that delivers strong and consistent surface staining in flow cytometry, enabling reliable discrimination of CD34-positive populations. Its signal intensity and low non-specific binding support clean population separation and facilitate integration into high-dimensional panels without compromising detection of co-expressed markers. This makes it particularly effective in studies requiring precise quantification of progenitor cell subsets.
Flow cytometry immunophenotyping using CD34 is widely applied in studies of bone marrow biology, immune reconstitution, and disease-associated alterations in progenitor populations. Changes in CD34-positive cell frequency, phenotype, or marker co-expression patterns provide insight into hematologic conditions and treatment responses. The ability to track these changes across experimental conditions makes CD34 an essential component of longitudinal and comparative immunophenotyping strategies.
In addition to hematopoietic systems, CD34 is also used to identify endothelial progenitor cells and vascular-associated populations in flow cytometry, expanding its relevance to angiogenesis and regenerative biology research. These applications further highlight the versatility of CD34 as a marker in multiparameter analysis.
Overall, CD34 Antibody for FACS enables high-resolution immunophenotyping of progenitor cell populations, supporting precise gating, multiparameter panel integration, and detailed analysis of hematopoietic and vascular cell systems.
This antibody is part of our CD34 antibody collection, supporting research into stem cell biology, endothelial markers, and tumor angiogenesis.
Optimal dilution of the CD34 Antibody for FACS / Flow Cytometry Immunophenotyping Antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Recombinant full-length human protein was used as the immunogen for the recombinant CD34 antibody.
Store the recombinant CD34 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
CD34 immunophenotyping antibody, CD34 flow cytometry marker antibody, hematopoietic progenitor marker antibody, CD34 multiparameter FACS antibody, CD34 lineage analysis antibody
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