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Home >> Antibodies >> Recombinant CD31 Antibody / Rabbit Monoclonal

Recombinant CD31 Antibody / Rabbit Monoclonal [clone C31/1395R] (V7243)

  Catalog No Formulation Size Price (USD)  
Image V7243-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V7243-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V7243SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V7243IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC staining of FFPE human angiocarcinoma with recombinant CD31 antibody (clone C31/1395R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human angiocarcinoma with recombinant CD31 antibody (clone C31/1395R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human ThP1 cell lysate with recombinant CD31 antibody (clone C31/1395R). Expected molecular weight: 83-130 kDa depending on level of glycosylation.
Flow cytometry testing of PFA-fixed human Jurkat cells with recombinant CD31 antibody (clone C31/1395R); Red=isotype control, Blue= recombinant CD31 antibody.
SDS-PAGE analysis of purified, BSA-free recombinant CD31 antibody (clone C31/1395R) as confirmation of integrity and purity.
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name C31/1395R
Purity Protein A affinity
UniProt P16284
Gene ID 5175
Localization Cell surface and cytoplasmic
Applications Western blot : 1-2ug/ml
Flow cytometry : 1-2ug/10^6 cells
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant CD31 antibody is available for research use only.
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Description

CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [RefSeq]

Application Notes

Titering of the recombinant CD31 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human full length recombinant protein was used as the immunogen for the recombinant CD31 antibody.

Storage

Store the recombinant CD31 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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