CD27 Antibody / Immune Memory Formation Marker Antibody [clone LPFS2/8607R] (V4234)

CD27 Antibody for WB. Western blot analysis of CD27 / TNFRSF7 expression in human Ramos B-cell lysate using CD27 Antibody / Immune Memory Formation Marker Antibody. Lane 1: Ramos cell lysate. A band is detected at approximately 50–60 kDa, consistent with glycosylated CD27, noting a lower predicted molecular weight of approximately 29 kDa for the core protein. CD27 is a glycosylated receptor, and the higher apparent molecular weight reflects N-linked glycosylation commonly observed in antigen-experienced lymphocyte populations. The detection of CD27 in Ramos cells aligns with its expression in B-cell lineage populations relevant to immune memory formation.

SDS-PAGE analysis of purified, BSA-free recombinant CD27 antibody (clone LPFS2/8607R) as confirmation of integrity and purity.

CD27 Antibody for IHC. Immunohistochemistry analysis of CD27 / TNFRSF7 expression in FFPE human tonsil tissue using CD27 Antibody / Immune Memory Formation Marker Antibody clone LPFS2/8607R. Strong membranous HRP-DAB brown staining is observed in lymphocyte populations within germinal centers and interfollicular regions, consistent with CD27 expression on memory T cells and memory B cells, while surrounding stromal elements remain largely negative. The staining pattern highlights immune memory compartments and supports evaluation of antigen-experienced lymphocyte populations within tonsillar tissue. Inset: PBS used in place of primary Ab (secondary Ab negative control). Heat-induced epitope retrieval was performed in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.