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Home >> Antibodies >> Recombinant CD27 Antibody

Recombinant CD27 Antibody [clone LPFS2/8607R] (V4234)

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  Catalog No Formulation Size Price (USD)  
Image V4234-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V4234-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V4234SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recrabbitmono
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Western blot analysis of Recombinant CD27 Antibody in human Ramos cell lysate. A prominent immunoreactive band is observed above the predicted molecular weight of approximately 29 kDa, consistent with glycosylated forms of CD27/TNFRSF7. CD27 is known to undergo N-linked glycosylation, which can result in slower migration and an apparent higher molecular weight on SDS-PAGE. The banding pattern in Ramos cells, a human B-cell line, supports specific detection of CD27 with expected post-translational modification-associated mobility shift.
SDS-PAGE analysis of purified, BSA-free recombinant CD27 antibody (clone LPFS2/8607R) as confirmation of integrity and purity.
IHC staining of FFPE human tonsil tissue with recombinant CD27 antibody (clone LPFS2/8607R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name LPFS2/8607R
Purity Protein A/G affinity
UniProt P26842
Localization Cell Surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Western Blot : 2-4ug/ml
Limitations This recombinant CD27 antibody is available for research use only.
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Description

CD27 antibody recognizes CD27, a member of the tumor necrosis factor receptor superfamily encoded by the CD27 gene. CD27 is a type I transmembrane glycoprotein primarily localized to the plasma membrane of T lymphocytes, memory B cells, and a subset of natural killer cells, where it functions as a co-stimulatory receptor involved in immune activation and survival signaling. Recombinant CD27 Antibody supports research focused on adaptive immunity, lymphocyte differentiation, and immune checkpoint biology.

The CD27 gene is located on chromosome 12p13 and encodes a protein containing extracellular cysteine-rich domains characteristic of the TNF receptor family, a single transmembrane region, and a cytoplasmic tail that interacts with TRAF signaling adaptors. CD27 binds its ligand CD70, leading to activation of NF-kappaB and other downstream pathways that promote T-cell expansion, memory formation, and B-cell maturation. CD27 expression is tightly regulated during lymphocyte activation and differentiation, making it a valuable marker for distinguishing naive and memory T-cell subsets.

CD27 is broadly expressed on naive and central memory T cells, as well as on memory B cells, but is downregulated in terminally differentiated effector populations. In lymphoid tissues such as tonsil and lymph node, CD27-positive cells are commonly observed within T-cell zones and germinal center-associated compartments. Dysregulated CD27 signaling has been implicated in chronic infections, autoimmune disorders, and lymphoid malignancies. Recombinant CD27 Antibody enables evaluation of CD27 distribution and expression dynamics in both normal immune responses and disease settings.

Because CD27-CD70 interactions influence immune activation and persistence, this pathway is actively investigated in cancer immunotherapy and immune modulation strategies. CD27 expression can provide insight into T-cell activation status and memory differentiation within tumor microenvironments. Clone LPFS2/8607R is engineered as a recombinant rabbit monoclonal antibody to provide consistent specificity and reproducibility in CD27 detection.

By targeting a key co-stimulatory receptor of adaptive immunity, Recombinant CD27 Antibody provides a reliable tool for studying lymphocyte biology, immune regulation, and translational immuno-oncology research.

Application Notes

Optimal dilution of the recombinant CD27 antibody should be determined by the researcher.

Immunogen

Recombinant human full-length protein was used as the immunogen for the recombinant CD27 antibody.

Storage

Aliquot the recombinant CD27 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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