CD27 Antibody / Tumor Immune Microenvironment Marker Antibody [clone LPFS2/8574R] (V4221)

CD27 Antibody for WB. Western blot analysis of CD27 / TNFRSF7 expression in human B-cell lymphoma cell lines using CD27 Antibody / Tumor Immune Microenvironment Marker Antibody. Lane 1: Raji cell lysate, Lane 2: Ramos cell lysate. A band is detected at approximately 50–60 kDa, consistent with glycosylated CD27, noting a lower predicted molecular weight of approximately 29 kDa for the core protein. Additional lower molecular weight signal may represent unmodified or partially processed forms of CD27. Detection of CD27 in Raji and Ramos cells aligns with expression in B-cell lineage populations relevant to tumor-associated immune environments.

CD27 Antibody for LN IHC. Immunohistochemistry analysis of CD27 / TNFRSF7 expression in FFPE human lymph node tissue using CD27 Antibody / Tumor Immune Microenvironment Marker Antibody clone LPFS2/8574R. Strong membranous HRP-DAB brown staining is observed in dense lymphocyte populations within nodal cortical and paracortical regions, consistent with CD27 expression on activated and antigen-experienced T and B cells, while non-lymphoid stromal elements remain largely negative. The staining pattern highlights immune cell distribution and supports evaluation of lymphocyte-rich microenvironments relevant to tumor-associated immune infiltration. Heat-induced epitope retrieval was performed in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

SDS-PAGE analysis of purified, BSA-free recombinant CD27 antibody (clone LPFS2/8574R) as confirmation of integrity and purity.