CD22 Antibody / B Cell Receptor Signaling Regulator Antibody [clone BLCAM/2637R] (V8594)

CD22 Antibody Human Tonsil IHC. Immunohistochemistry analysis of CD22/Siglec-2 expression in FFPE human tonsil tissue using recombinant CD22 Antibody / B Cell Receptor Signaling Regulator Antibody clone BLCAM/2637R. Strong HRP-DAB brown membranous and cytoplasmic staining is observed in germinal center and mantle zone B lymphocytes, highlighting dense B cell populations involved in antigen-driven immune responses, while surrounding stromal and non-lymphoid cells remain largely negative. The staining pattern reflects CD22 function as a regulator of B cell receptor signaling and supports use of this CD22 antibody for immunohistochemistry-based evaluation of B cell activation and signaling context in lymphoid tissues. Heat-induced epitope retrieval was performed in pH 9 Tris-EDTA buffer for 20 minutes followed by cooling prior to antibody incubation.

CD22 Antibody Human Spleen IHC. Immunohistochemistry analysis of CD22/Siglec-2 expression in FFPE human spleen tissue using CD22 Antibody / B Cell Receptor Signaling Regulator Antibody clone BLCAM/2637R. Strong HRP-DAB brown staining is localized to B lymphocyte-rich regions within the white pulp, with prominent membranous and cytoplasmic signal highlighting follicular B cell populations, while surrounding red pulp and non-lymphoid elements remain largely negative. The staining distribution is consistent with CD22-mediated regulation of B cell receptor signaling in splenic immune compartments and supports use of this CD22 antibody for immunohistochemistry-based assessment of lymphoid tissue organization and signaling context. Heat-induced epitope retrieval was performed in pH 9 Tris-EDTA buffer for 20 minutes followed by cooling prior to antibody incubation.