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Home >> Antibodies >> Recombinant CD21 Antibody

Recombinant CD21 Antibody [clone rCR2/6964] (V4773)

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  Catalog No Formulation Size Price (USD)  
Image V4773-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V4773-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V4773SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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CD21 Antibody B-cell Line WB. Western blot analysis of Complement Receptor 2 / CD21 in human spleen, Daudi, Raji, and Ramos lysates using recombinant mouse monoclonal CD21 antibody, clone rCR2/6964. A band is detected at approximately 20–25 kDa in all samples, consistent with the expected molecular weight of CD21 fragments, with strong signal observed in B-cell lymphoma lines reflecting CD21 expression in B-cell populations.
SDS-PAGE analysis of purified, BSA-free recombinant CD21 antibody (clone rCR2/6964) as confirmation of integrity and purity.
CD21 Antibody Placenta IHC. Immunohistochemistry of Complement Receptor 2 / CD21 in FFPE human placenta tissue using recombinant CD21 antibody, clone rCR2/6964. HRP-DAB brown staining highlights trophoblast cell membranes lining chorionic villi, while stromal cores show minimal staining and nuclei are counterstained blue. Inset: PBS was used in place of primary antibody as a negative control to confirm specificity of staining.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name rCR2/6964
Purity Protein A/G affinity
UniProt P20023
Localization Cell surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Western Blot : 2-4ug/ml
Limitations This recombinant CD21 antibody is available for research use only.
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Description

Recognizes a protein of 140kDa, which is identified as the complement receptor 2 (CR2) or CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFN alpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.

For a validated reference of CD21 expression across B cells and follicular dendritic cell networks, see CD21 antibody clone CR2/2754 with supporting multi-application data.

Application Notes

Optimal dilution of the recombinant CD21 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 900-1033) from the human protein was used as the immunogen for the recombinant CD21 antibody.

Storage

Aliquot the recombinant CD21 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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