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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
CD21 Antibody for IF / Complement Receptor 2 Immunofluorescence Antibody [clone CR2/3124R] (V7754)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V7754-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
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V7754-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
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V7754SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | CR2/3124R |
| Purity | Protein A affinity chromatography |
| UniProt | P20023 |
| Localization | Cell surface |
| Applications | Flow Cytometry : 1-2ug/million cells in 0.1ml Immunofluorescence : 1-2ug/ml Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml |
| Limitations | This CD21 Antibody for IF / Complement Receptor 2 Immunofluorescence Antibody is available for research use only. |
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Complement receptor 2 (CR2), also known as CD21, is a cell surface glycoprotein that functions as part of the B-cell co-receptor complex and plays a central role in antigen recognition and immune activation. CD21 Antibody for IF / Complement Receptor 2 Immunofluorescence Antibody is optimized for fluorescence-based detection of CD21, enabling visualization of its distribution in cellular systems. CD21 antibody, also referred to as CR2 antibody in the literature, is widely used in studies of B-cell biology, immune signaling, and host-pathogen interactions.
CD21 is primarily expressed on mature B lymphocytes and follicular dendritic cells, where it binds complement fragments such as C3d to enhance B-cell receptor signaling. In addition to its role in adaptive immunity, CD21 serves as the receptor for Epstein-Barr virus, mediating viral attachment and entry into B cells. These functions make CD21 an important marker for both normal immune processes and disease-related mechanisms.
In immunofluorescence applications, CD21 is typically observed as membrane-associated and punctate fluorescence corresponding to cell surface localization and receptor clustering. This allows detailed visualization of CD21 distribution at the single-cell level and supports studies of receptor organization and cellular heterogeneity. Fluorescence-based detection also enables co-localization studies with other immune markers, providing insight into complex signaling environments.
Immunofluorescent staining of human MOLT4 cells demonstrates detectable CD21 signal under the conditions tested, with green fluorescence marking CD21-positive regions and nuclear counterstaining providing cellular context. This supports the use of CD21 antibody for IF in cell-based assays where spatial resolution and subcellular localization are important.
In addition to immunofluorescence, this antibody is compatible with complementary applications including immunohistochemistry and flow cytometry, allowing consistent detection of CD21 across experimental platforms. Flow cytometry enables quantitative assessment of CD21-positive populations, while immunohistochemistry supports visualization of tissue-level expression patterns in lymphoid structures.
The recombinant rabbit monoclonal clone CR2/3124R antibody provides reliable fluorescence signal and consistent performance in IF applications. Its ability to visualize CD21 distribution at the cellular level makes it well suited for studies of B-cell activation, receptor localization, and immune cell characterization.
For a validated reference of CD21 expression across B cells and follicular dendritic cell networks, see CD21 antibody clone CR2/2754 with supporting multi-application data.
Optimal dilution of the CD21 Antibody for IF / Complement Receptor 2 Immunofluorescence Antibody should be determined by the researcher.
A partial protein corresponding to amino acids 142-240 was used as the immunogen for the recombinant CD21 antibody.
Store the CD21 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
CR2 antibody, Complement receptor 2 antibody, CD21 IF antibody, B-cell receptor CD21 antibody, Epstein-Barr virus receptor antibody
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