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Home >> Antibodies >> Recombinant CD19 Antibody Microarray Validated

Recombinant CD19 Antibody Microarray Validated [clone CD19/4260R] (V5821)

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  Catalog No Formulation Size Price (USD)  
Image V5821-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V5821-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V5821SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recrabbitmono
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant CD19 antibody (clone CD19/4260R). These results demonstrate the foremost specificity of the CD19/4260R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
IHC staining of FFPE human tonsil tissue with recombinant CD19 antibody (clone CD19/4260R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human tonsil tissue lysate with recombinant CD19 antibody (clone CD19/4260R). Expected molecular weight: 60~100 kDa depending on glycosylation level.
Western blot testing of human Raji cell lysate with recombinant CD19 antibody (clone CD19/4260R). Expected molecular weight: 60~100 kDa depending on glycosylation level.
SDS-PAGE analysis of purified, BSA-free recombinant CD19 antibody (clone CD19/4260R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG1, kappa
Clone Name CD19/4260R
UniProt P15391
Localization Cell surface, cytoplasmic
Applications Western Blot : 2-4ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant CD19 antibody is available for research use only.
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Description

CD19 antibody recognizes CD19, a B cell-specific type I transmembrane glycoprotein that functions as a critical coreceptor in B cell receptor signaling. CD19 is a member of the immunoglobulin superfamily and is expressed from the early pro-B cell stage through mature peripheral B lymphocytes, where it localizes to the plasma membrane. By forming a signaling complex with CD21, CD81, and CD225, CD19 amplifies antigen receptor-mediated signaling and lowers the threshold for B cell activation. Recombinant CD19 Antibody Microarray Validated is designed for consistent detection of CD19 in high-throughput tissue microarray platforms and other research applications requiring reproducible performance.

The CD19 gene is located on chromosome 16p11.2 and encodes a protein containing two extracellular immunoglobulin-like domains, a single transmembrane segment, and a cytoplasmic tail enriched in tyrosine residues that become phosphorylated upon activation. Through recruitment of signaling molecules such as PI3K, CD19 contributes to downstream pathways controlling B cell proliferation, differentiation, and survival. Recombinant CD19 Antibody Microarray Validated supports investigations into normal B cell development as well as dysregulated signaling in disease states.

In normal human tissues, CD19 expression is restricted to B lineage cells within bone marrow, lymph node, tonsil, and spleen. Terminally differentiated plasma cells typically exhibit reduced or absent CD19 expression, reflecting changes during late-stage B cell maturation. Because of this lineage specificity, CD19 serves as a reliable pan-B cell marker in immunologic research and translational studies.

Aberrant CD19 expression is characteristic of most B cell malignancies, including B cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and numerous non-Hodgkin lymphomas. CD19 has also emerged as a major therapeutic target in immunotherapy strategies, including chimeric antigen receptor T cell approaches. Accurate detection of CD19 is therefore important for studies of tumor biology, immune targeting, and treatment response.

Clone CD19/4260R is a recombinant rabbit monoclonal antibody engineered for high specificity and lot-to-lot consistency. Recombinant production ensures defined sequence identity and stable performance across experimental batches. Recombinant CD19 Antibody Microarray Validated clone CD19/4260R produces strong membranous staining in CD19-positive B cells with minimal background in non-B cell populations, making it well suited for research applications that require precise and reproducible antigen detection.

Application Notes

Optimal dilution of the recombinant CD19 antibody should be determined by the researcher.

Immunogen

A recombinant fragment of human CD19 protein (around aa1-300) was used as the immunogen for the recombinant CD19 antibody.

Storage

Aliquot the recombinant CD19 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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