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Home >> Antibodies >> Recombinant CD146 Antibody

Recombinant CD146 Antibody [clone RM249] (R20269)

  Catalog No Formulation Size Price (USD)  
Image R20269-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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Western blot testing of human A375 cell lysate with recombinant CD146 antibody at 1:1000. Observed molecular weight 70-120 kDa depending on glycosylation level.
IHC testing of FFPE human breast cancer tissue with recombinant CD146 antibody at 1:400.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM249
Purity Protein A purified from animal origin-free supernatant
UniProt P43121
Gene ID 4162
Applications Immunohistochemistry (FFPE) : 1:200-1:500 (1)
Western blot : 1:1000-1:2000
Limitations This recombinant CD146 antibody is available for research use only.
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Description

This recombinant CD146 antibody reacts to human CD146 (Cell surface glycoprotein MUC18). It may also react to the mouse and rat protein, as predicted by immunogen homology.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant CD146 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A peptide corresponding to the residues near C-terminus of human CD146 was used as the immunogen for this recombinant CD146 antibody.

Storage

Store the recombinant CD146 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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