TNFRSF9 Antibody 4-1BB/4552R Recombinant CD137

	Catalog No	Formulation	Size	Price (USD)
	V8061-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	559
Formulation
	V8061-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	259
Formulation
	V8061SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation

TNFRSF9 Antibody 4-1BB/4552R Staining in Human Kidney IHC. Immunohistochemistry staining of FFPE human kidney using TNFRSF9 Antibody (clone 4-1BB/4552R) shows strong brown staining associated predominantly with renal tubular epithelial structures, with heterogeneous staining intensity across the tissue. HIER was performed by boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling before testing. The staining demonstrates detection of TNFRSF9 using this Recombinant CD137 Antibody in human kidney.

TNFRSF9 Antibody 4-1BB/4552R Staining in Human Kidney IHC. Immunohistochemistry staining of a second FFPE human kidney specimen using TNFRSF9 Antibody (clone 4-1BB/4552R) shows prominent brown staining outlining numerous renal tubules, while surrounding areas display comparatively weaker signal. HIER was performed by boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling before testing. This second kidney specimen provides additional tissue-based detection of TNFRSF9 using the Recombinant CD137 Antibody.

TNFRSF9 Antibody 4-1BB/4552R Detects CD137 in Human Kidney WB. Western blot analysis of human kidney lysate using TNFRSF9 Antibody (clone 4-1BB/4552R) shows a strong, discrete band at approximately 50-55 kDa with minimal visible background. CD137 has a predicted monomeric molecular weight near 30 kDa, but the receptor is glycosylated and can migrate at substantially higher apparent molecular weights. The observed band is therefore consistent with detection of CD137 using this Recombinant CD137 Antibody.

TNFRSF9 Antibody 4-1BB/4552R Detects CD137 Across Human Cell Lysates WB. Western blot analysis of human kidney, HEK293, K-562, Raji, and Ramos lysates using TNFRSF9 Antibody (clone 4-1BB/4552R) shows a prominent band at approximately 45-50 kDa across all five samples. Signal intensity is relatively consistent among the lysates, with little visible nonspecific background. The higher apparent molecular weight relative to the predicted CD137 monomer is compatible with glycosylated receptor species and demonstrates reproducible detection across multiple human sample types using this Recombinant CD137 Antibody.

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TNFRSF9 Antibody 4-1BB/4552R Staining in Human Kidney IHC. Immunohistochemistry staining of FFPE human kidney using TNFRSF9 Antibody (clone 4-1BB/4552R) shows strong brown staining associated predominantly with renal tubular epithelial structures, with heterogeneous staining intensity across the tissue. HIER was performed by boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling before testing. The staining demonstrates detection of TNFRSF9 using this Recombinant CD137 Antibody in human kidney.

TNFRSF9 Antibody 4-1BB/4552R Staining in Human Kidney IHC. Immunohistochemistry staining of a second FFPE human kidney specimen using TNFRSF9 Antibody (clone 4-1BB/4552R) shows prominent brown staining outlining numerous renal tubules, while surrounding areas display comparatively weaker signal. HIER was performed by boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling before testing. This second kidney specimen provides additional tissue-based detection of TNFRSF9 using the Recombinant CD137 Antibody.

TNFRSF9 Antibody 4-1BB/4552R Detects CD137 in Human Kidney WB. Western blot analysis of human kidney lysate using TNFRSF9 Antibody (clone 4-1BB/4552R) shows a strong, discrete band at approximately 50-55 kDa with minimal visible background. CD137 has a predicted monomeric molecular weight near 30 kDa, but the receptor is glycosylated and can migrate at substantially higher apparent molecular weights. The observed band is therefore consistent with detection of CD137 using this Recombinant CD137 Antibody.

TNFRSF9 Antibody 4-1BB/4552R Detects CD137 Across Human Cell Lysates WB. Western blot analysis of human kidney, HEK293, K-562, Raji, and Ramos lysates using TNFRSF9 Antibody (clone 4-1BB/4552R) shows a prominent band at approximately 45-50 kDa across all five samples. Signal intensity is relatively consistent among the lysates, with little visible nonspecific background. The higher apparent molecular weight relative to the predicted CD137 monomer is compatible with glycosylated receptor species and demonstrates reproducible detection across multiple human sample types using this Recombinant CD137 Antibody.

Availability	1-3 business days
Species Reactivity	Human
Format	Purified
Host	Mouse
Clonality	Recombinant Rabbit Monoclonal
Isotype	Rabbit IgG, kappa
Clone Name	4-1BB/4552R
Purity	Protein A affinity chromatography
UniProt	Q07011
Localization	Cell surface, cytoplasmic
Applications	Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Western Blot : 2-4ug/ml
Limitations	This TNFRSF9 Antibody 4-1BB/4552R / Recombinant CD137 Antibody is available for research use only.
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Description

TNFRSF9 Antibody 4-1BB/4552R recognizes tumor necrosis factor receptor superfamily member 9 (TNFRSF9), an immune costimulatory receptor more commonly known as CD137 or 4-1BB. TNFRSF9 is a member of the tumor necrosis factor receptor superfamily and is induced following activation of several immune-cell populations. The receptor is particularly associated with activated T lymphocytes and is also expressed by natural killer cells and other immune cells involved in innate and adaptive immune responses.

TNFRSF9 interacts with its ligand TNFSF9, also known as CD137L or 4-1BBL. Ligand engagement promotes recruitment of TRAF-family signaling proteins to the receptor and activates downstream pathways that include NF-kappaB and MAPK signaling. These signals can promote lymphocyte survival, proliferation, cytokine production, and effector activity. The CD137 signaling axis therefore provides an important mechanism for regulating the magnitude and persistence of cellular immune responses.

TNFRSF9 expression and signaling are studied extensively in immunology, inflammation, and cancer research. Because receptor expression increases following immune-cell activation, CD137 is frequently investigated in studies of activated lymphocyte populations and tumor-associated immune responses. Modulation of 4-1BB signaling is also of considerable interest in cancer immunology because of its ability to influence T-cell and natural killer cell activity. A Recombinant CD137 Antibody can complement studies examining receptor expression and the biological mechanisms associated with CD137-mediated immune costimulation.

Clone 4-1BB/4552R is a recombinant rabbit monoclonal antibody generated against a human CD137 protein fragment within amino acids 19-188. Its recombinant monoclonal format provides a defined reagent for investigating TNFRSF9 expression and complements antibodies produced using conventional monoclonal formats. NSJ Bioreagents supplies clone 4-1BB/4552R for research use only.

A TNFRSF9 Antibody can be used to investigate CD137 expression and its relationship to lymphocyte activation, immune costimulation, inflammatory responses, and antitumor immunity.

For a CD137 monoclonal with broader IF, FACS, IHC, Western blot, and protein microarray validation, see our CD137 Antibody 4-1BB/3201 / 4-1BB Antibody page.

Application Notes

Optimal dilution of the TNFRSF9 Antibody 4-1BB/4552R / Recombinant CD137 Antibody should be determined by the researcher.

Immunogen

A human CD137 protein fragment within amino acids 19-188 was used as the immunogen for the recombinant CD137 / TNFRSF9 antibody.

Storage

Store the TNFRSF9 Antibody 4-1BB/4552R at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

CD137 antibody, 4-1BB antibody, 4-1BBR antibody, tumor necrosis factor receptor superfamily member 9 antibody, CDw137 antibody, ILA antibody

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TNFRSF9 Antibody 4-1BB/4552R / Recombinant CD137 Antibody [clone 4-1BB/4552R] (V8061)

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NSJ Bioreagents