CD11b C-Terminal Antibody RM290 / Recombinant CD11b Antibody [clone RM290] (R20316)

CD11b C-Terminal Antibody RM290 Western Blot of HL60 Cells. Western blot testing of untreated (-) and DMSO-treated (+) human HL60 cell lysates with clone RM290 at 1:400 dilution. A strong band is detected at approximately 160-170 kDa, within the expected 127-170 kDa range for glycosylated CD11b. The blot shows substantially greater CD11b signal in the DMSO-treated sample than in the untreated sample, consistent with increased CD11b expression following differentiation of HL60 cells. The C-terminal-directed epitope of this recombinant CD11b antibody provides an additional distinction from antibodies directed against other regions of the protein.

CD11b C-Terminal Antibody RM290 in Human Tonsil IHC. Immunohistochemistry testing of formalin-fixed paraffin-embedded human tonsil tissue with clone RM290 at 1:200 dilution. Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 min followed by cooling before staining. The image shows widespread membranous and cytoplasmic staining among immune cells within the tonsillar tissue, with heterogeneous staining intensity across the field. This staining demonstrates FFPE tissue detection using the recombinant CD11b antibody.