Cathepsin D Antibody / CTSD [clone CTSD/9613R] (V5441)

Cathepsin D Antibody Pancreatic Tissue IHC. Immunohistochemistry analysis of FFPE human pancreas tissue using recombinant Cathepsin D antibody (clone CTSD/9613R) shows strong granular cytoplasmic staining throughout pancreatic cells, consistent with CTSD / Cathepsin D localization in lysosomal compartments. The punctate staining pattern reflects lysosomal protease distribution associated with intracellular protein processing and turnover. Tissue architecture is well preserved, and surrounding stromal regions display comparatively lower staining. Hematoxylin counterstain provides nuclear contrast and structural context. HIER: boil FFPE tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Cathepsin D antibody (clone CTSD/9613R) as confirmation of integrity and purity.

Cathepsin D Antibody Human Tissue Panel WB. Western blot analysis of human 1) brain, 2) heart, 3) skeletal muscle, 4) skin, and 5) pancreas tissue lysates using Cathepsin D Antibody (clone CTSD/9613R) detects a consistent band at approximately 30-35 kDa across all samples, corresponding to the processed mature form of Cathepsin D (CTSD). Cathepsin D is synthesized as a larger precursor that undergoes proteolytic cleavage to generate active heavy and light chain fragments, with the observed band reflecting the predominant mature species. The uniform banding pattern across tissues supports reliable detection of CTSD and is consistent with its widespread lysosomal expression.