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Home >> Antibodies >> Recombinant Carcinoembryonic Antigen Antibody / CEA

Recombinant Carcinoembryonic Antigen Antibody / CEA [clone C66/6470R] (V9318)

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  Catalog No Formulation Size Price (USD)  
Image V9318-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9318-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9318SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human colon tissue with recombinant Carcinoembryonic Antigen antibody (clone C66/6470R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human colon tissue with recombinant Carcinoembryonic Antigen antibody (clone C66/6470R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue using recombinant Carcinoembryonic Antigen antibody (clone C66/6470R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Carcinoembryonic Antigen antibody (clone C66/6470R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name C66/6470R
Purity Protein A/G affinity
UniProt P06731
Localization Cytoplasm and luminal surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant Carcinoembryonic Antigen antibody is available for research use only.
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Description

This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.

Application Notes

Optimal dilution of the recombinant Carcinoembryonic Antigen antibody should be determined by the researcher.

Immunogen

Recombinant full-length human CEA protein was used as the immunogen for the recombinant Carcinoembryonic Antigen antibody.

Storage

Aliquot the recombinant Carcinoembryonic Antigen antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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