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Home >> Antibodies >> Recombinant Calponin Antibody / Rabbit Monoclonal

Recombinant Calponin Antibody / Rabbit Monoclonal [clone CNN1/1408R] (V7248)

  Catalog No Formulation Size Price (USD)  
Image V7248-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V7248-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V7248SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V7248IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC testing of FFPE human uterus stained with recombinant Calponin antibody (clone CNN1/1408R). Required HIER: steam sections in 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE rat uterus stained with recombinant Calponin antibody (clone CNN1/1408R). Required HIER: steam sections in 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of permeabilized human K562 cells with recombinant Calponin antibody (green, clone CNN1/1408R) and Reddot nuclear stain (red).
Flow cytometry testing of PFA-fixed human K562 cells with recombinant Calponin antibody (clone CNN1/1408R); Red=isotype control, Blue= recombinant Calponin antibody.
SDS-PAGE analysis of purified, BSA-free recombinant Calponin antibody (clone CNN1/1408R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name CNN1/1408R
Purity Protein A affinity chromatography
UniProt P51911
Localization Cytoplasmic
Applications Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This recombinant Calponin antibody is available for research use only.
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Description

Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction.

Application Notes

Optimal dilution of the recombinant Calponin antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the recombinant Calponin antibody.

Storage

Store the recombinant Calponin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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