Caldesmon Antibody / CALD1 Cellular Mechanical Stress Response Protein Antibody [clone rCALD1/820] (V8878)

Caldesmon Antibody / CALD1 Cellular Mechanical Stress Response Protein Antibody. Western blot analysis of Caldesmon (CALD1) in human ovary tissue lysate. Lane 1: human ovary tissue lysate. A band is detected at approximately 120-150 kDa, consistent with the predicted molecular weight of Caldesmon / CALD1 and representing the high molecular weight h-caldesmon isoform associated with mechanically resilient contractile cells. Additional lower molecular weight bands may be observed at approximately 70-80 kDa corresponding to non-muscle isoforms involved in cytoskeletal adaptation to mechanical stress. The observed banding pattern reflects isoform-dependent expression of CALD1 linked to cellular mechanical stress response and cytoskeletal stability.

Caldesmon Antibody / CALD1 Cellular Mechanical Stress Response Protein Antibody. Immunohistochemistry analysis of Caldesmon (CALD1) in human leiomyosarcoma tissue. FFPE human leiomyosarcoma stained with Caldesmon Antibody, clone rCALD1/820, demonstrates strong HRP-DAB brown cytoplasmic staining in tumor cells with smooth muscle differentiation. The staining highlights densely packed, spindle-shaped tumor cells arranged in intersecting fascicles, consistent with cytoskeletal reinforcement and mechanical stress-adapted structural organization. Prominent staining in vascular smooth muscle structures further supports localization of CALD1 to mechanically resilient contractile cell populations. Heat-induced epitope retrieval was performed using Tris-EDTA buffer at pH 9.

SDS-PAGE analysis of purified, BSA-free recombinant Caldesmon antibody (clone rCALD1/820) as confirmation of integrity and purity.