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Home >> Antibodies >> Recombinant Cadherin 16 Antibody

Recombinant Cadherin 16 Antibody [clone CDH16/9611R] (V5384)

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  Catalog No Formulation Size Price (USD)  
Image V5384-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V5384-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V5384SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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Recombinant Cadherin 16 Antibody Kidney WB. Western blot testing of human kidney tissue lysate using recombinant Cadherin 16 antibody (clone CDH16/9611R).
Recombinant Cadherin 16 Antibody Kidney IHC. Immunohistochemical analysis of Cadherin-16 / CDH16 expression in formalin-fixed, paraffin-embedded human kidney tissue using CDH16 antibody. Strong membranous and luminal staining is observed in renal tubular epithelial cells, with clear visualization of tubular structures and glomerular regions consistent with kidney-specific expression of CDH16.
SDS-PAGE analysis of purified, BSA-free recombinant Cadherin 16 antibody (clone CDH16/9611R) as confirmation of integrity and purity.
Cadherin 16 Antibody Human Kidney WB. Western blot analysis of Cadherin-16 / CDH16 expression across kidney tissue lysates using Cadherin 16 antibody clone CDH16/9611R. Lane 1: human kidney lysate, Lane 2: mouse kidney lysate, Lane 3: rat kidney lysate. A strong band is detected at approximately 95–110 kDa in human kidney lysate, consistent with the predicted molecular weight of Cadherin-16 (CDH16), with the slightly higher apparent migration reflecting glycosylation of this kidney-restricted adhesion protein. Minimal or no detectable signal is observed in mouse and rat kidney lysates under these conditions, supporting preferential detection in human tissue.
Cadherin 16 Antibody Human and Rat Kidney WB. Western blot analysis of Cadherin-16 / CDH16 expression in kidney tissue lysates using Cadherin 16 antibody clone CDH16/9611R. Lane 1: human kidney lysate, Lane 2: rat kidney lysate. A strong band is detected at approximately 100-110 kDa in human kidney lysate, consistent with the predicted molecular weight of Cadherin-16 (CDH16), with the slightly higher apparent migration reflecting glycosylation of this kidney-restricted adhesion protein. Minimal or no detectable signal is observed in rat kidney lysate under these conditions, indicating preferential detection in human tissue.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name CDH16/9611R
Purity Protein A/G affinity
UniProt O75309
Localization Cell Surface, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 2-4ug/ml
Limitations This recombinant Cadherin 16 antibody is available for research use only.
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Description

This MAb recognizes a protein of 130kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels. Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.

This antibody is part of a broader antibody panel offered by NSJ Bioreagents.

Application Notes

Optimal dilution of the recombinant Cadherin 16 antibody should be determined by the researcher.

Immunogen

A recombinant fragment (within amino acids 350-550) of human CDH16 protein was used as the immunogen for the recombinant Cadherin 16 antibody.

Storage

Aliquot the recombinant Cadherin 16 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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