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Home >> Antibodies >> Recombinant Cadherin 16 Antibody / CDH16

Recombinant Cadherin 16 Antibody / CDH16 [clone CDH16/7027R] (V9442)

  Catalog No Formulation Size Price (USD)  
Image V9442-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 499
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V9442-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 219
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V9442SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 499
IHC staining of FFPE human thyroid tissue with recombinant Cadherin 16 antibody (clone CHD16/7027R) at 2ug/ml in PBS for 30min RT. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney tissue with recombinant Cadherin 16 antibody (clone CDH16/7027R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney tissue with recombinant Cadherin 16 antibody (clone CHD16/7027R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Cadherin 16 antibody (clone CDH16/7027R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name CDH16/7027R
Purity Protein A affinity
UniProt O75309
Localization Cell surface, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant Cadherin 16 antibody is available for research use only.
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Description

This MAb recognizes a protein of 130kDa, identified as Ksp-cadherin/cadherin 16/CDH16. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels.Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.

Application Notes

Optimal dilution of the recombinant Cadherin 16 antibody should be determined by the researcher.

Immunogen

Recombinant human full-length protein was used as the immunogen for the recombinant Cadherin 16 antibody.

Storage

Aliquot the recombinant Cadherin 16 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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