CAD Antibody / Caldesmon CALD1 Actin Cytoskeleton Organization Antibody [clone CALD1/7024R] (V9428)

CAD Antibody / Caldesmon CALD1 Actin Cytoskeleton Organization Antibody. Western blot analysis of Caldesmon (CALD1) in human ovary tissue lysate. Lane 1: human ovary tissue lysate. A band is detected at approximately 70-80 kDa, consistent with the predicted molecular weight of Caldesmon / CALD1 and representing the lower molecular weight non-muscle isoform. Additional higher molecular weight bands may be observed at approximately 120-150 kDa corresponding to the smooth muscle-associated h-caldesmon isoform. CALD1 is known to produce multiple isoforms with distinct migration patterns, and the observed banding profile reflects isoform-dependent differences associated with cytoskeletal organization in muscle and non-muscle cells.

CAD Antibody / Caldesmon CALD1 Actin Cytoskeleton Organization Antibody. Immunohistochemistry analysis of Caldesmon (CALD1) in human colon tissue. FFPE human colon stained with CAD Antibody, clone CALD1/7024R, demonstrates strong HRP-DAB brown cytoplasmic staining in smooth muscle cells of the muscularis layer. The staining highlights elongated, spindle-shaped cells with dense filamentous cytoplasmic signal consistent with organized actin cytoskeleton structures and contractile filament bundles. Surrounding epithelial and stromal compartments show minimal staining, supporting localization of CALD1 to cytoskeletal networks within smooth muscle cells. Heat-induced epitope retrieval was performed using Tris-EDTA buffer at pH 9.

SDS-PAGE analysis of purified, BSA-free recombinant CAD antibody (clone CALD1/7024R) as confirmation of integrity and purity.