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Home >> Antibodies >> Recombinant CAD Antibody / CALD1 / Caldesmon

Recombinant CAD Antibody / CALD1 / Caldesmon [clone CALD1/7024R] (V9428)

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  Catalog No Formulation Size Price (USD)  
Image V9428-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9428-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9428SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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Western blot testing of human ovary tissue lysate using recombinant CAD antibody (clone CALD1/7024R). Predicted molecular weight ~93 kDa, can be observed at 70-80 kDa (non muscle tissue) and 120-150 kDa (smooth muscle).
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Western blot testing of human ovary tissue lysate using recombinant CAD antibody (clone CALD1/7024R). Predicted molecular weight ~93 kDa, can be observed at 70-80 kDa (non muscle tissue) and 120-150 kDa (smooth muscle).
IHC staining of FFPE human colon tissue with recombinant CAD antibody (clone CALD1/7024R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CAD antibody (clone CALD1/7024R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name CALD1/7024R
Purity Protein A/G affinity
UniProt Q05682
Localization Cytoplasm
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant CAD antibody is available for research use only.
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Description

Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells.

Application Notes

Optimal dilution of the recombinant CAD antibody should be determined by the researcher.

Immunogen

Recombinant human full-length Caldesmon protein was used as the immunogen for the recombinant CAD antibody.

Storage

Aliquot the recombinant CAD antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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