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Home >> Antibodies >> Recombinant BRCA1-Associated Protein 1 Antibody / BAP1

Recombinant BRCA1-Associated Protein 1 Antibody / BAP1 [clone BAP1/9120R] (V5608)

  Catalog No Formulation Size Price (USD)  
Image V5608-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5608-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5608SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
Microvalidated Recrabbitmono
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IHC staining of FFPE human breast carcinoma tissue with recombinant BRCA1-Associated Protein 1 antibody (clone BAP1/9120R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast carcinoma tissue with recombinant BRCA1-Associated Protein 1 antibody (clone BAP1/9120R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name BAP1/9120R
Purity Protein A/G affinity
UniProt Q92560
Localization Nucleus, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant BRCA1-Associated Protein 1 antibody is available for research use only.
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Description

The BAP1 gene belongs to the ubiquitin C-terminal hydrolase subfamily of de-ubiquitination enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germ line mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, Uveal melanoma and cutaneous melanoma.

Application Notes

Optimal dilution of the recombinant BRCA1-Associated Protein 1 antibody should be determined by the researcher.

Immunogen

A recombinant fragment (within amino acids 500-729) of human BAP1 protein was used as the immunogen for the recombinant BRCA1-Associated Protein 1 antibody.

Storage

Aliquot the recombinant BRCA1-Associated Protein 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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