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Home >> Antibodies >> Recombinant Brain Creatine Kinase Antibody / CKBB / CKB

Recombinant Brain Creatine Kinase Antibody / CKBB / CKB [clone CKBB/8310R] (V4376)

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  Catalog No Formulation Size Price (USD)  
Image V4376-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4376-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4376SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human brain tissue with recombinant Brain Creatine Kinase antibody (clone CKBB/8310R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human brain tissue with recombinant Brain Creatine Kinase antibody (clone CKBB/8310R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Brain Creatine Kinase antibody (clone CKBB/8310R) as confirmation of integrity and purity.
Western blot testing of human HEK293 cell lysate with recombinant Brain Creatine Kinase antibody (clone CKBB/8310R). Predicted molecular weight ~43 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name CKBB/8310R
Purity Protein A/G affinity
UniProt P12277
Localization Cytoplasm
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant Brain Creatine Kinase antibody is available for research use only.
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Description

Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This mAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.

Application Notes

Optimal dilution of the recombinant Brain Creatine Kinase antibody should be determined by the researcher.

Immunogen

Recombinant human full-length protein was used as the immunogen for the recombinant Brain Creatine Kinase antibody.

Storage

Aliquot the recombinant Brain Creatine Kinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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