BRAF V600E Antibody RM8 / BRAF V600E Mutation Antibody [clone RM8] (R20306)

BRAF V600E Antibody RM8 Detection of Mutant and Wild-Type BRAF WB. Western blot analysis of cell lysates expressing endogenous BRAF V600E or wild-type BRAF using BRAF V600E Antibody clone RM8. A distinct band is detected at approximately 85 kDa in the V600E sample, while no corresponding signal is evident in the wild-type sample, supporting mutation-specific recognition of BRAF V600E.

BRAF V600E Antibody RM8 Staining of Human Melanoma IHC. Immunohistochemistry staining of FFPE human melanoma tissue using BRAF V600E Antibody clone RM8. Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 min followed by cooling. Strong brown immunoreactivity is visible throughout the tumor-cell population, with predominantly cytoplasmic staining.

BRAF V600E Antibody RM8 Staining of V600E-Positive Thyroid Carcinoma IHC. Immunohistochemistry staining of FFPE human thyroid carcinoma confirmed positive for the BRAF V600E mutation by allele-specific PCR using BRAF V600E Antibody clone RM8. Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 min followed by cooling. Strong, widespread brown immunoreactivity is evident within the carcinoma, consistent with detection of mutant BRAF V600E protein.

BRAF V600E Antibody RM8 Testing of V600E-Negative Thyroid Carcinoma IHC. Immunohistochemistry staining of FFPE human thyroid carcinoma confirmed negative for the BRAF V600E mutation by allele-specific PCR using BRAF V600E Antibody clone RM8. Following heat-induced epitope retrieval in pH 6 citrate buffer, the tissue shows little to no specific brown staining, supporting discrimination between V600E-positive and V600E-negative thyroid carcinoma.

BRAF V600E Antibody RM8 Staining of V600E-Positive WiDr Colon Cancer Cells ICC. Immunocytochemistry staining of FFPE human WiDr colorectal adenocarcinoma cells using BRAF V600E Antibody clone RM8. Heat-induced epitope retrieval was performed by steaming in pH 6 citrate buffer for 20 min followed by cooling. Strong brown immunoreactivity is evident in the positive cell population, demonstrating detection of BRAF V600E under the tested conditions.

BRAF V600E Antibody RM8 Testing of V600E-Negative SW40 Cells ICC. Immunocytochemistry staining of FFPE human SW40 cells using BRAF V600E Antibody clone RM8. Heat-induced epitope retrieval was performed by steaming in pH 6 citrate buffer for 20 min followed by cooling. Little to no specific brown staining is evident in this negative cell sample, providing a contrasting control for the BRAF V600E-positive WiDr cells.