Recombinant Beta Catenin Antibody [clone rCTNNB1/2173] (V3601)

IHC testing of FFPE human breast cancer with recombinant beta Catenin antibody (clone rCTNNB1/2173). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant beta Catenin antibody (clone rCTNNB1/2173) as confirmation of integrity and purity.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant beta Catenin antibody (clone rCTNNB1/2173). These results demonstrate the foremost specificity of the rCTNNB1/2173 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Western blot testing of human HeLa cell lysate with recombinant beta Catenin antibody. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.

Western blot testing of human brain tissue lysate with recombinant beta Catenin antibody. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.

Immunofluorescent staining of human HeLa cells with recombinant beta Catenin antibody (clone rCTNNB1/2173, green) and Reddot nuclear stain (red).