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Home >> Antibodies >> Recombinant BAX Antibody

Recombinant BAX Antibody [clone BAX/3158R] (V5783)

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  Catalog No Formulation Size Price (USD)  
Image V5783-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5783-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5783SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
Microvalidated Recrabbitmono
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Western blot testing of human A549 and MCF7 cell lysate with recombinant BAX antibody (clone BAX/3158R). Predicted molecular weight ~21 kDa.
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Western blot testing of human A549 and MCF7 cell lysate with recombinant BAX antibody (clone BAX/3158R). Predicted molecular weight ~21 kDa.
Western blot testing of human A549 cell lysate with recombinant BAX antibody (clone BAX/3158R). Predicted molecular weight ~21 kDa.
SDS-PAGE analysis of purified, BSA-free recombinant BAX antibody (clone BAX/3158R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name BAX/3158R
UniProt Q07812
Localization Cytoplasm
Applications Western Blot : 2-4ug/ml
Limitations This recombinant BAX antibody is available for research use only.
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Description

Recognizes a protein of 21 kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

Application Notes

Optimal dilution of the recombinant BAX antibody should be determined by the researcher.

Immunogen

A portion of amino acids 1-100 from human BAX protein was used as the immunogen for the recombinant BAX antibody.

Storage

Aliquot the recombinant BAX antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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