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Home >> Antibodies >> Recombinant Basic Cytokeratin Antibody

Recombinant Basic Cytokeratin Antibody [clone rKRTH/8883] (V4282)

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  Catalog No Formulation Size Price (USD)  
Image V4282-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4282-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4282SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human skin tissue with recombinant Basic Cytokeratin antibody (clone rKRTH/8883). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human skin tissue with recombinant Basic Cytokeratin antibody (clone rKRTH/8883). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2b, kappa
Clone Name rKRTH/8883
Purity Protein A/G affinity
UniProt Q01546
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant Basic Cytokeratin antibody is available for research use only.
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Description

This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 5048, 46, 45, and 40kDa. mAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Application Notes

Optimal dilution of the recombinant Basic Cytokeratin antibody should be determined by the researcher.

Immunogen

Recombinant full-length human KRT76 protein was used as the immunogen for the recombinant Basic Cytokeratin antibody.

Storage

Aliquot the recombinant Basic Cytokeratin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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