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Home >> Antibodies >> Recombinant Aurora B Antibody

Recombinant Aurora B Antibody [clone RM278] (R20295)

  Catalog No Formulation Size Price (USD)  
Image R20295-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 379
IHC testing of FFPE human tonsil tissue with recombinant Aurora B antibody at 1:1000.
Western blot of HeLa cell lysate, untreated (-) or treated (+) with Nocodazole (NOC), using recombinant Aurora B antibody at 1:1000. Predicted molecular weight: 39~45 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM278
Purity Protein A purified from animal origin-free supernatant
UniProt Q96GD4
Gene ID 9212
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 1:200-1:500 (1)
Western blot : 1:1000-1:2000
Limitations This recombinant Aurora B antibody is available for research use only.
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Description

This recombinant Aurora B antibody reacts to human Aurora kinase B.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Aurora B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A peptide corresponding to the N-terminus of human Aurora kinase B was used as the immunogen for this recombinant Aurora B antibody.

Storage

Store the recombinant Aurora B antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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