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Home >> Antibodies >> Recombinant AURKB Antibody / Aurora B

Recombinant AURKB Antibody / Aurora B [clone ARRK2-2R] (V7296)

  Catalog No Formulation Size Price (USD)  
Image V7296-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 459
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V7296-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 209
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V7296SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 459
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V7296IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 459
IHC testing of FFPE human colon tissue with recombinant AURKB antibody (clone ARRK2-2R). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human. Other species not tested.
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name ARRK2-2R
Purity Protein A affinity
UniProt Q96GD4
Localization Nuclear, cytoplasmic
Applications IHC (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant AURKB antibody is available for research use only.
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Description

Recognizes a protein of 39kDa, which is identified as Aurora B. The serine/threonine protein kinase aurora B (Aurora B) is a chromosomal passenger protein critical for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors.

Application Notes

Optimal dilution of the recombinant AURKB antibody should be determined by the researcher.

Immunogen

A portion of amino acids 89-251 was used as the immunogen for this recombinant AURKB antibody.

Storage

Store the recombinant AURKB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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