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Home >> Antibodies >> Recombinant Anti-Kappa Antibody / Rabbit Monoclonal

Recombinant Anti-Kappa Antibody / Rabbit Monoclonal [clone IGKC/1999R] (V3591)

  Catalog No Formulation Size Price (USD)  
Image V3591-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 459
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V3591-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 209
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V3591SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 459
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V3591IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 459
IHC testing of FFPE human tonsil tissue with recombinant anti-Kappa antibody (clone IGKC/1999R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant anti-Kappa antibody (clone IGKC/1999R) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant anti-Kappa antibody (clone IGKC/1999R). These results demonstrate the foremost specificity of the IGKC/1999R mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name IGKC/1999R
Purity Protein A affinity chromatography
UniProt P01601 , P01834
Localization Cell Surface, cytoplasmic and secreted
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant anti-Kappa antibody is available for research use only.
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Description

This mAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Application Notes

The optimal dilution of the recombinant anti-Kappa antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human Ig kappa chain was used as the immunogen for this recombinant anti-Kappa antibody.

Storage

Store the recombinant anti-Kappa antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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