Amyloid A4 Antibody APP/9971R / A4 Antibody [clone APP/9971R] (V5746)

Amyloid A4 Antibody APP/9971R in Human Brain and U-87 MG Lysates WB. Western blot testing of human brain and U-87 MG cell lysates with Amyloid A4 Antibody clone APP/9971R detects a prominent band at approximately 110-120 kDa in both samples, with minimal additional reactivity. The observed molecular weight is consistent with mature APP, which undergoes post-translational modification including glycosylation. The discrete high-molecular-weight signal demonstrates recognition by the A4 Antibody in human brain and cultured human cells.

Purified Amyloid A4 Antibody APP/9971R Reducing and Non-Reducing SDS-PAGE. SDS-PAGE analysis of purified, BSA-free Amyloid A4 Antibody clone APP/9971R shows prominent heavy and light antibody chains at approximately 50 and 25 kDa under reducing conditions. Under non-reducing conditions, the predominant intact antibody species migrates at approximately 150 kDa, with little additional protein staining visible. The gel demonstrates the expected molecular composition and integrity of the purified A4 Antibody preparation.

Amyloid A4 Antibody APP/9971R in Human Brain Tissue IHC. Immunohistochemistry staining of FFPE human brain tissue with Amyloid A4 Antibody clone APP/9971R demonstrates heterogeneous brown staining within the brain parenchyma, including cytoplasmic and neuropil-associated signal. HIER was performed by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling before testing. The PBS negative-control inset shows little to no corresponding brown staining, supporting the specificity of the observed A4 Antibody signal under the tested conditions.