- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
ALPP Antibody Recombinant Mouse MAb rALP/870 / Placental Alkaline Phosphatase [clone rALP/870] (V9465)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V9465-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V9465-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V9465SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | rALP/870 |
| Purity | Protein A/G affinity |
| UniProt | P05187 |
| Localization | Cytoplasmic, Cell surface |
| Applications | Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml |
| Limitations | This ALPP antibody is available for research use only. |
|
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Alkaline phosphatase placental is a glycosylphosphatidylinositol-anchored membrane enzyme encoded by the ALPP gene and commonly referred to as placental alkaline phosphatase or PLAP. The ALPP Antibody Recombinant Mouse MAb rALP/870 is developed to detect this trophoblast-associated alkaline phosphatase in tissue-based research applications. ALPP is located on chromosome 2q37 and belongs to the alkaline phosphatase family of homodimeric metalloenzymes that catalyze the hydrolysis of phosphate monoesters under alkaline conditions.
In normal physiology, PLAP is strongly expressed on the apical membrane of syncytiotrophoblasts within placental chorionic villi. Because the enzyme is anchored to the external surface of the plasma membrane through a glycosylphosphatidylinositol linkage, immunohistochemical detection typically demonstrates distinct membranous staining in trophoblastic cells. Most non-placental adult tissues exhibit minimal expression, supporting its role as a lineage-associated marker in histologic studies of placental differentiation.
In oncologic research, ALPP expression has been widely evaluated in germ cell tumors, particularly seminoma and embryonal carcinoma, as well as in certain trophoblastic neoplasms. Tumor epithelial cells in these contexts frequently demonstrate strong membranous and occasional cytoplasmic staining, whereas most non-germ cell carcinomas show little to no staining. This relatively restricted distribution pattern enhances its value in research panels assessing germ cell lineage and trophoblastic differentiation within tissue sections.
Although placental alkaline phosphatase shares structural similarity with tissue-nonspecific and intestinal alkaline phosphatase isoenzymes, its trophoblastic and germ cell-associated expression profile is more defined. Clone rALP/870 is a recombinant mouse monoclonal antibody developed for specific detection of ALPP in formalin-fixed, paraffin-embedded specimens and other research applications involving membrane-associated enzyme localization.
Optimal dilution of the ALPP antibody should be determined by the researcher.
Recombinant full-length human ALPP protein was used as the immunogen for the recombinant ALPP antibody recombinant mouse mAb rALP/870.
Aliquot the ALPP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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