- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
ALPP Antibody Rabbit Monoclonal ALPP/9109R / Placental Alkaline Phosphatase [clone ALPP/9109R] (V5430)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V5430-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
|
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V5430-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V5430SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | ALPP/9109R |
| Purity | Protein A/G affinity |
| UniProt | P05187 |
| Localization | Cytoplasm, Cell membrane |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml |
| Limitations | This ALPP antibody is available for research use only. |
|
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Alkaline phosphatase placental is a glycosylphosphatidylinositol-anchored membrane enzyme encoded by the ALPP gene and widely known as placental alkaline phosphatase or PLAP. The ALPP Antibody Rabbit Monoclonal ALPP/9109R is developed to detect this trophoblast-associated alkaline phosphatase in research applications focused on tissue distribution and tumor-associated expression. ALPP is located on chromosome 2q37 and belongs to the alkaline phosphatase family of homodimeric metalloenzymes that catalyze the hydrolysis of phosphate monoesters under alkaline conditions.
In normal physiology, PLAP is strongly expressed on the apical membrane of syncytiotrophoblasts in placental chorionic villi. Because the enzyme is attached to the external surface of the plasma membrane through a glycosylphosphatidylinositol anchor, immunohistochemical detection typically demonstrates distinct membranous staining in trophoblastic cells, while stromal elements and most other adult tissues show minimal expression. This restricted distribution supports its use as a lineage-associated marker in tissue-based studies.
In oncologic research, ALPP expression has been extensively examined in germ cell tumors, particularly seminoma and embryonal carcinoma, as well as in certain trophoblastic neoplasms. Strong membranous and occasional cytoplasmic staining patterns are commonly observed in tumor epithelial cells in these settings, whereas most non-germ cell carcinomas display little to no staining. This differential expression profile enhances its value in studies evaluating germ cell differentiation and trophoblastic lineage markers within histologic architecture.
Although placental alkaline phosphatase shares structural similarity with tissue-nonspecific and intestinal alkaline phosphatase isoenzymes, its trophoblastic and germ cell-associated expression pattern is more defined. Clone ALPP/9109R is a rabbit monoclonal antibody developed for specific detection of ALPP in formalin-fixed, paraffin-embedded specimens and other research applications involving membrane-associated enzyme localization.
Optimal dilution of the recombinant ALPP antibody ALPP/9109R should be determined by the researcher.
Recombinant full-length human Placental Alkaline Phosphatase protein was used as the immunogen for the recombinant ALPP antibody rabbit monoclonal ALPP/9109R.
Aliquot the ALPP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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