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Home >> Antibodies >> Aldehyde Dehydrogenase 1A1 Antibody / ALDH1A1

Aldehyde Dehydrogenase 1A1 Antibody / ALDH1A1 [clone ALDH1A1/7011R] (V9491)

  Catalog No Formulation Size Price (USD)  
Image V9491-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V9491-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V9491SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recrabbitmono
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Aldehyde Dehydrogenase 1A1 Antibody Brain Immunohistochemistry. IHC staining of FFPE human brain tissue with Aldehyde Dehydrogenase 1A1 antibody (clone ALDH1A1/7011R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Aldehyde Dehydrogenase 1A1 Antibody Kidney Immunohistochemistry. IHC staining of FFPE human kidney tissue with recombinant Aldehyde Dehydrogenase 1A1 antibody (clone ALDH1A1//7011R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Aldehyde Dehydrogenase 1A1 antibody (clone ALDH1A1/7011R) as confirmation of integrity and purity.
Aldehyde Dehydrogenase 1A1 Antibody Human Brain Tissue WB. Western blot testing of human brain tissue lysate using recombinant Aldehyde Dehydrogenase 1A1 antibody (clone ALDH1A1/7011R). Expected molecular weight ~55 kDa.
ALDH1A1 Antibody Multi-Species Liver Tissue WB. Western blot analysis of human liver, mouse liver, rat liver, hamster liver, and guinea pig liver tissue lysates using Aldehyde dehydrogenase 1A1 antibody clone ALDH1A1/7011R. A prominent band is detected at approximately 55 kDa in all liver samples, consistent with the predicted molecular weight of Aldehyde dehydrogenase 1 family member A1 / ALDH1A1. The broadly conserved banding pattern across multiple mammalian species aligns with the known hepatic expression and detoxification-associated function of this cytosolic aldehyde metabolism enzyme.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Hamster, Rabbit, Guinea pig
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name ALDH1A1/7011R
Purity Protein A/G affinity
UniProt P00352
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml (Human)
Western Blot : 2-4ug/ml (Human/Mouse/Rat/Hamster/Rabbit/Guinea pig
Limitations This Aldehyde Dehydrogenase 1A1 antibody is available for research use only.
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Description

Aldehyde Dehydrogenase 1A1 Antibody detects ALDH1A1, belonging to the ALDH enzymes, a family of evolutionarily conserved enzymes comprised of 19 isoforms that are localized in the cytoplasm, mitochondria or nucleus. ALDH1A1 is predominantly expressed in the epithelium of testis, brain, eye, liver, kidney, as well as neural and hematopoietic stem cells. Reportedly, high ALDH1A1 expression is found in solitary fibrous tumor (SFT) and hemangiopericytoma (HPC), compared to meningiomas and synovial sarcomas. In combination with CD34, ALDH1A1 may be useful for the differentiation among SFT, HPC, meningioma, and synovial sarcoma.

For broader multi-application ALDH1A1 detection including IF and flow cytometry workflows, see our ALDH1A1 Antibody / Cancer Stem Cell and Retinoic Acid Metabolism Marker page.

Application Notes

Optimal dilution of the Aldehyde Dehydrogenase 1A1 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 315-434 was used as the immunogen for the recombinant Aldehyde Dehydrogenase 1A1 antibody.

Storage

Aliquot the Aldehyde Dehydrogenase 1A1 ALDH1A1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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