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Home >> Antibodies >> Recombinant AKT1 Antibody

Recombinant AKT1 Antibody [clone RM252] (R20273)

  Catalog No Formulation Size Price (USD)  
Image R20273-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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Western blot testing of 293 cell lysate with recombinant AKT1 antibody at 1:1000. Predicted molecular weight ~56 kDa.
IHC testing of FFPE human breast cancer tissue with recombinant AKT1 antibody at 1:1000.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Bovine, Mouse, Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM252
Purity Protein A purified from animal origin-free supernatant
UniProt P31749
Gene ID 207
Applications Immunohistochemistry (FFPE) : 1:500-1:1000 (1)
Western blot : 1:1000-1:2000
Limitations This recombinant AKT1 antibody is available for research use only.
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Description

This recombinant AKT1 antibody may also react to bovine, mouse or rat AKT1, as predicted by immunogen homology

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant AKT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A peptide corresponding to the C-terminus of human Akt1 was used as the immunogen for this recombinant AKT1 antibody.

Storage

Store the recombinant AKT1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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