- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Recombinant AKT1 Antibody [clone AKT1/3898R] (V8442)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
 |
V8442-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 519 | |
|
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V8442-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 229 | |
|
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V8442SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 519 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | AKT1/3898R |
| Purity | Protein A affinity chromatography |
| UniProt | P31749 |
| Localization | Nuclear, cytoplasmic, cell surface |
| Applications | Western blot : 1-2ug/ml ELISA : order Ab without BSA for coating |
| Limitations | This recombinant AKT1 antibody is available for research use only. |
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Recognizes a protein of 62kDa, which is identified as AKT1. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.
Optimal dilution of the recombinant AKT1 antibody should be determined by the researcher.
A portion of amino acids 85-189 from the human protein was used as the immunogen for the recombinant AKT1 antibody.
Store the recombinant AKT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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