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Home >> Antibodies >> Recombinant ACTH Antibody (Synacthen)

Recombinant ACTH Antibody (Synacthen) [clone rCLIP/3814] (V8807)

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  Catalog No Formulation Size Price (USD)  
Image V8807-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V8807-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V8807SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human pituitary gland tissue with recombinant ACTH antibody (clone rCLIP/3814) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pituitary gland tissue with recombinant ACTH antibody (clone rCLIP/3814) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse and Rat (broad species reactivity predicted)
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rCLIP/3814
Purity Protein A/G affinity
UniProt P01189
Localization Cytoplasm, Secreted
Applications ELISA (For coating order BSA-free format) :
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant ACTH antibody is available for research use only.
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Description

ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin.

Application Notes

Optimal dilution of the recombinant ACTH antibody should be determined by the researcher.

Immunogen

A recombinant protein fragment from the N-terminal region of human ACTH was used as the immunogen for the recombinant ACTH antibody.

Storage

Aliquot the recombinant ACTH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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