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Home >> Antibodies >> Recombinant ACTH Antibody (CLIP polypeptide)

Recombinant ACTH Antibody (CLIP polypeptide) [clone rCLIP/1407] (V8938)

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  Catalog No Formulation Size Price (USD)  
Image V8938-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V8938-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V8938SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human pituitary with recombinant ACTH antibody (clone rCLIP/1407). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human pituitary with recombinant ACTH antibody (clone rCLIP/1407). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pituitary tissue with recombinant ACTH antibody (clone rCLIP/1407). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant ACTH antibody (clone rCLIP/1407) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Mouse and Rat (broad species reactivity predicted)
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rCLIP/1407
Purity Protein A/G affinity
UniProt P01189
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant ACTH antibody is available for research use only.
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Description

POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.

Application Notes

Optimal dilution of the recombinant ACTH antibody should be determined by the researcher.

Immunogen

Amino acids NGAEDESAEAFPLEF from human ACTH was used as the immunogen for the recombinant ACTH antibody. This sequence is from the CLIP or Corticotropin-like intermediary peptide polypeptide of ACTH.

Storage

Aliquot the recombinant ACTH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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