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Home >> Antibodies >> Recombinant ACTC1 Antibody

Recombinant ACTC1 Antibody [clone RM257] (R20278)

  Catalog No Formulation Size Price (USD)  
Image R20278-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 379
Western blot testing of mouse heart lysate with recombinant ACTC1 antibody at 1:1000. Predicted molecular weight ~42 kDa.
IHC testing of FFPE human heart tissue with recombinant ACTC1 antibody at 1:1000.
Availability 1-3 business days
Species Reactivity Human, Mouse
Predicted Reactivity Bovine, Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM257
Purity Protein A purified from animal origin-free supernatant
UniProt P68032
Gene ID 70
Applications Immunohistochemistry (FFPE) : 1:1000-1:2000 (1)
Western blot : 1:1000-1:2000
Limitations This recombinant ACTC1 antibody is available for research use only.
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Description

The recombinant ACTC1 antibody reacts to human and mouse alpha cardiac actin (Actin, alpha cardiac muscle 1). It may also react to bovine or rat alpha-cardiac actin, as predicted by immunogen homology.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant ACTC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A peptide corresponding to the N-terminus of human alpha-cardiac Actin was used as the immunogen for this recombinant ACTC1 antibody.

Storage

Store the recombinant ACTC1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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