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Home >> Antibodies >> Recombinant ACTA2 Antibody

Recombinant ACTA2 Antibody [clone RM253] (R20272)

  Catalog No Formulation Size Price (USD)  
Image R20272-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 379
Western blot testing of mouse heart lysate with recombinant ACTA2 antibody at 1:1000. Predicted molecular weight ~42 kDa.
IHC testing of FFPE human heart tissue with recombinant ACTA2 antibody at 1:2500.
IHC testing of FFPE human kidney tissue with recombinant ACTA2 antibody at 1:2500.
Availability 1-3 business days
Species Reactivity Human, Mouse
Predicted Reactivity Bovine, Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM253
Purity Protein A purified from animal origin-free supernatant
UniProt P62736
Gene ID 59
Applications Immunohistochemistry (FFPE) : 1:1000-1:2500 (1)
Western blot : 1:1000-1:2000
Limitations This recombinant ACTA2 antibody is available for research use only.
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Description

This recombinant Alpha Smooth Muscle Actin / ACTA2 antibody reacts to human and mouse alpha smooth muscle Actin (alpha-Actin-2). It may also react to bovine or rat alpha smooth muscle Actin, as predicted by immunogen homology.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant ACTA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A peptide corresponding to the N-terminus of human alpha smooth muscle Actin was used as the immunogen for this recombinant ACTA2 antibody.

Storage

Store the recombinant ACTA2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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