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Home >> Antibodies >> Recombinant Acidic Cytokeratin Antibody (LMW / Type I)

Recombinant Acidic Cytokeratin Antibody (LMW / Type I) [clone rKRTL/6616] (V9300)

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  Catalog No Formulation Size Price (USD)  
Image V9300-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9300-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9300SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human sebaceous gland with recombinant Acidic Cytokeratin antibody (clone rKRTL/6616). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human sebaceous gland with recombinant Acidic Cytokeratin antibody (clone rKRTL/6616). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human skin tissue with recombinant Acidic Cytokeratin antibody (clone rKRTL/6616). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Acidic Cytokeratin antibody (clone rKRTL/6616) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rKRTL/6616
Purity Protein A/G affinity
UniProt Q7Z794
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant Acidic Cytokeratin antibody is available for research use only.
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Description

The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK10, CK14, CK15 and CK16 and low molecular weight CK19. Its usually used in a cytokeratin cocktail with type II cytokeratin antibody.

Application Notes

Optimal dilution of the recombinant Acidic Cytokeratin antibody should be determined by the researcher.

Immunogen

A synthetic peptide sequence common to Type I Cytokeratins was used as the immunogen for the recombinant Acidic Cytokeratin antibody.

Storage

Aliquot the recombinant Acidic Cytokeratin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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