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Home >> Antibodies >> Recombinant Acetylated Alpha Tubulin Antibody / TUBA4A (K40)

Recombinant Acetylated Alpha Tubulin Antibody / TUBA4A (K40) [clone RM318] (R20337)

  Catalog No Formulation Size Price (USD)  
Image R20337-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 379
Western blot of extracts from human HeLa cells, untreated (-) or treated (+) with Trichostatin A (TSA), using the recombinant Acetylated Alpha Tubulin antibody at 1:1000.
IF/ICC testing of human HeLa cells, untreated (-) or treated (+) with Trichostatin A, with recombinant Acetylated Alpha Tubulin antibody (Red) at 1:5000. Green = actin fillaments, Blue = DAPI nuclear counterstain.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM318
Purity Protein A purified from animal origin-free supernatant
UniProt P68366
Applications Western Blot : 1:1000-1:2000
Immunofluorescence/Immunocytochemistry : 1:1000-1:5000
Limitations This recombinant Acetylated Alpha Tubulin antibody is available for research use only.
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Description

The acetylated residue of alpha tubulin is K40, which is catalyzed by alpha tubulin acetyl-transferase (α-TAT) in humans. The acetylation of K40 on alpha tubulin is a hallmark of stable microtubules. [Wiki]

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Acetylated Alpha Tubulin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An acetyl-peptide corresponding to the K40 region of human alpha tubulin was used as the immunogen for the recombinant Acetylated Alpha Tubulin antibody.

Storage

Store the recombinant Acetylated Alpha Tubulin antibody at -20oC.

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