RBP1 Antibody / Retinol Binding Protein 1 [clone RBP/872] (V2828)

Description

Recognizes a protein of 21kDa-25kDa, identified as retinol binding protein-1 (RBP1). This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein post-transnationally and results in defective delivery and supply to the epidermal cells.

Application Notes

Optimal dilution of the RBP1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human Retinol binding protein-1 was used as the immunogen for the RBP1 antibody.

Storage

Store the RBP1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).