- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Rbfox3 Antibody / Fox3 / Neun (RQ6896)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ6896 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity purified |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | Q8BIF2 |
| Localization | Nucleus, Cytoplasm |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence : 5ug/ml Direct ELISA : 0.1-0.5ug/ml |
| Limitations | This Rbfox3 antibody is available for research use only. |
|
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Rbfox3 antibody recognizes RNA binding protein fox-1 homolog 3, widely known as Fox3 and NeuN, a neuron-specific RNA binding protein encoded by the RBFOX3 gene. NeuN serves as a robust marker for postmitotic neurons and is expressed throughout the central and peripheral nervous systems. The human RBFOX3 gene is located on chromosome 17q25.3 and is part of the Rbfox family of splicing regulators, which also includes RBFOX1 and RBFOX2. Fox3 protein localizes primarily to neuronal nuclei, with some cytoplasmic distribution depending on developmental stage, cell type, and isoform expression. High expression is found in cortical neurons, hippocampal pyramidal cells, cerebellar granule cells, brainstem nuclei, and many sensory neuron populations.
NeuN functions as a regulator of alternative splicing in neurons. Through its RNA recognition motif, it binds UGCAUG elements in target transcripts and modulates splicing patterns involved in neuronal differentiation, synaptogenesis, and activity-dependent responses. Rbfox family proteins participate in pathways that influence ion channel diversity, synaptic strength, axon guidance, and neuronal survival. NeuN plays key roles in establishing the mature neuronal transcriptome by supporting the inclusion or exclusion of exons required for neuronal excitability, cytoskeletal organization, and membrane trafficking. Its consistent expression in postmitotic neurons makes it a cornerstone marker in neurodevelopmental, neurodegenerative, and injury-related research.
At the subcellular level, Fox3/NeuN predominantly localizes to the nucleus where it co-localizes with splicing machinery components, transcriptional regulators, and nuclear matrix structures. Some isoforms display partial cytoplasmic localization and may influence mRNA stability or translation. Fox3/NeuN is absent from certain neuronal populations, including Purkinje cells, olfactory receptor neurons, and retinal photoreceptors, highlighting cell-type specific expression. During development, RBFOX3 expression increases as neural progenitors exit the cell cycle and differentiate into mature neurons. This developmental trajectory mirrors the shift toward neuron-specific splicing programs that define identity and connectivity.
NeuN is highly relevant in disease research. Loss or mislocalization of RBFOX3 has been associated with neurodegenerative disorders, traumatic brain injury, epilepsy, and neurodevelopmental syndromes. Perturbation of Rbfox splicing networks can alter synaptic components, ion channel expression, and cortical circuitry. Reduced NeuN immunoreactivity is frequently used as an indicator of neuronal loss in experimental models of stroke, excitotoxicity, and neuroinflammation. In cancer biology, RBFOX3 expression has been implicated in neuronal differentiation states within neuroblastoma and related tumors.
This Rbfox3 antibody is suitable for detecting NeuN expression in research focused on neuronal identity, brain development, splicing regulation, neurodegeneration, circuitry mapping, and central nervous system injury models. It supports studies examining neuronal population dynamics, transcriptome maturation, and tissue architecture within both developing and adult nervous systems. NSJ Bioreagents provides this reagent within its neuroscience-focused antibody portfolio.
Optimal dilution of the Rbfox3 antibody should be determined by the researcher.
Recombinant mouse protein (amino acids H28-D304) was used as the immunogen for the Rbfox3 antibody.
After reconstitution, the Rbfox3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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