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Home >> Antibodies >> Rat EpCAM Antibody

Rat EpCAM Antibody [clone Epcam/1159] (V3118S)

  Catalog No Formulation Size Price (USD)  
Image V3118S-0.5ML Bioreactor concentrate with 0.05% sodium azide 0.5 ml 519
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V3118S-0.1ML Bioreactor concentrate with 0.05% sodium azide 100 ul 229
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V3118IHC-7ML Prediluted in 1X PBS, 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC: Formalin-fixed, paraffin-embedded rat lung stained with EpCAM antibody (Epcam/1159).
IHC: Formalin-fixed, paraffin-embedded rat colon stained with EpCAM antibody (Epcam/1159).
Availability 1-3 business days
Species Reactivity Rat, Mouse
Format Bioreactor concentrate
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name Epcam/1159
Purity Unpurified high titer supernatant
UniProt O55159
Localization Cell surface, cytoplasmic
Applications Immunohistochemistry (FFPE) : 1:50-1:100 for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (3)
Limitations This rat EpCAM antibody is available for research use only.
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Description

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.

Application Notes

Optimal dilution of the rat EpCAM antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The epitopes of mAbs Epcam/1158 and Epcam/1159 monoclonal antibodies are different.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant full-length rat protein was used as the immunogen for the rat EpCAM antibody.

Storage

Store the rat EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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