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Home >> Antibodies >> RagD Antibody / RRAGD

RagD Antibody / RRAGD (F54410)

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  Catalog No Formulation Size Price (USD)  
Image F54410-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54410-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human MDA-MB-435 cell lysate with RagD antibody. Predicted molecular weight: ~46 kDa (isoform 1) and ~29 kDa (isoform 2).
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Western blot testing of human MDA-MB-435 cell lysate with RagD antibody. Predicted molecular weight: ~46 kDa (isoform 1) and ~29 kDa (isoform 2).
Western blot testing of human 293T cell lysate with RagD antibody. Predicted molecular weight: ~46 kDa (isoform 1) and ~29 kDa (isoform 2).
Western blot testing of mouse cerebellum lysate with RagD antibody. Predicted molecular weight: ~51 kDa and ~30 kDa (multiple isoforms).
IHC testing of FFPE human brain tissue with RagD antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human MDA-MB-435 cells with RagD antibody; Blue=isotype control, Green= RagD antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q9NQL2
Localization Nuclear, cytoplasmic
Applications Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Western blot : 1:500-1:2000
Limitations This RagD antibody is available for research use only.
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Description

Ras-related GTP-binding protein D is a monomeric guanine nucleotide-binding protein, or G protein. By binding GTP or GDP, small G proteins act as molecular switches in numerous cell processes and signaling pathways.

Application Notes

The stated application concentrations are suggested starting points. Titration of the RagD antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 171-199 from the human protein was used as the immunogen for the RagD antibody. Isoforms 1 and 2 may be detected by this RagD antibody.

Storage

Aliquot the RagD antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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