RAG1 Antibody / Recombination activating gene 1 (RQ6151)

RAG1 Antibody Human T Cell and Mouse Thymus WB. Western blot analysis was performed using human Jurkat T cell lysate in lane 1 and mouse thymus tissue lysate in lane 2. The RAG1 antibody detected a distinct band near the predicted molecular weight of approximately 120 kDa in both samples. Detection in Jurkat cells and thymus tissue supports recognition of human and mouse RAG1 in lymphoid samples. RAG1 Antibody, recognizing recombination activating gene 1, supports research into VDJ recombination, antigen receptor formation and developing lymphocyte biology.

RAG1 Antibody Human and Mouse Cell and Thymus WB. Western blot analysis was performed under reducing conditions using 30 ug of lysate per lane. Lane 1: human Jurkat cells; Lane 2: human K562 cells; Lane 3: human A431 cells; Lane 4: mouse thymus tissue; Lane 5: mouse RAW264.7 cells. The membrane was incubated overnight at 4°C with rabbit anti-RAG1 antibody at 0.5 ug/ml, followed by an HRP-conjugated secondary antibody and ECL detection. A specific band was observed at approximately 119 kDa, matching the expected size of RAG1. RAG1 Antibody, recognizing recombination activating gene 1, supports studies of VDJ recombination and lymphocyte development.