- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
RAF1 Antibody / c-Raf (F54952)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
 |
F54952-0.4ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.4 ml | 399 | |
|
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F54952-0.08ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.08 ml | 185 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Purity | Antigen affinity purified |
| UniProt | P04049 |
| Localization | Cytoplasmic, nuclear |
| Applications | Flow cytometry : 1:10-1:50 (1x10e6 cells) Immunofluorescence : 1:10-1:50 Immunohistochemistry (FFPE) : 1:50-1:100 Western blot : 1:500-1:1000 |
| Limitations | This RAF1 antibody is available for research use only. |
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RAF1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, RAF1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
The stated application concentrations are suggested starting points. Titration of the RAF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 1-30 from the human protein was used as the immunogen for the RAF1 antibody.
Aliquot the RAF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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