RAD51 Antibody / DNA Repair Protein Antibody [clone RAD51/2702] (V7334)

RAD51 Antibody Colon Carcinoma IHC. Immunohistochemical staining of FFPE human colon carcinoma demonstrates RAD51 expression using a RAD51 antibody. RAD51, also known as RAD51 Recombinase, is a DNA repair protein that mediates homologous recombination and preserves genomic stability following DNA damage. Predominantly nuclear staining is observed in tumor cells, consistent with the role of RAD51 in DNA double strand break repair and the elevated DNA repair activity frequently seen in proliferating cancers. Heat induced epitope retrieval was performed by boiling tissue sections in pH6 10 mM citrate buffer for 10 to 20 minutes followed by cooling prior to staining. These results support the utility of RAD51 Antibody for investigating DNA damage responses, homologous recombination, and therapeutic resistance in human malignancies.

RAD51 Antibody Protein Microarray Validation. Analysis of a HuProt human protein microarray containing more than 19,000 full length human proteins demonstrates the exceptional specificity of the RAD51 antibody clone RAD51/2702. RAD51, also known as RAD51 Recombinase, is a key DNA repair protein involved in homologous recombination and maintenance of genomic stability. Among all proteins represented on the array, RAD51 exhibited the highest signal intensity with a Z score of 85.84 and an S score of 82.68, indicating strong and highly selective target recognition. The Z score reflects the signal strength generated by antibody binding relative to the mean signal of all proteins on the array, while the S score measures the difference between the top ranked target and the next highest signal. These results support the foremost specificity of the RAD51/2702 mAb and validate its utility for studies of DNA damage responses, genome maintenance, and cancer biology.

RAD51 Antibody K562 Cells FACS. Flow cytometric analysis of human K562 cells was performed using RAD51 antibody (clone RAD51/2702) followed by Goat anti-Mouse IgG-CF488 secondary antibody. RAD51, also known as RAD51 Recombinase, is a key mediator of homologous recombination and DNA double strand break repair that maintains genomic stability and coordinates cellular responses to DNA damage. Blue histogram: RAD51 antibody. Red histogram: isotype control. The marked rightward shift of the RAD51 antibody signal relative to the isotype control demonstrates specific detection of endogenous RAD51 in K562 cells. These results support the utility of this antibody for flow cytometric studies of DNA repair pathways, genome maintenance, and cellular responses to genotoxic stress.